Generator
Part:BBa_J100136:Design
Designed by: Malcolm Campbell Group: Campbell M Lab (2014-02-20)
T5+riboswitchD+thyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 100
Illegal AgeI site found at 763 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used golden gate assembly to put the thyA gene into T5+riboswitch into pSB1K8 plasmid. We used BsaI restriction enzyme.
Source
composite device from existing parts